合肥市售鸡蛋壳表面与内容物沙门氏菌污染的 检测及其分离株耐药性分析

Detection of Salmonella from the commercial egg shell and egg contents and analysis of its drug resistance in Hefei area

中文关键词:  鸡蛋  蛋壳表面  蛋内容物  沙门氏菌  耐药性

英文关键词:egg  egg shell surface  egg contents  Salmonella  drug resistance

中文摘要:

      目的 了解市售鸡蛋壳表面、内容物中沙门氏菌的污染情况及分离菌株的血清型分布与耐药性。方法 采用沙门氏菌常规检测法并结合PCR技术, 对采自合肥市农贸市场、超市的100枚鸡蛋进行检测; 利用国标法和K-B 纸片法对分离株进行血清型鉴定和抗生素药物敏感试验。结果 蛋壳表面沙门氏菌检出率为10%(10/100), 蛋内容物沙门氏菌检出率为1%(1/100); 11株沙门氏菌分离株血清型均为鸭沙门氏菌; 14种抗生素药物敏感试验中, 对青霉素100%耐受, 链霉素18.2%耐药, 36.4%中等耐药率, 其余抗生素均敏感。 结论 合肥市售鸡蛋壳表面的沙门氏菌检出率高于蛋内容物, 抗生素选择压力导致不同地区沙门氏菌分离株的血清型和耐药性有所不同。

英文摘要:

      Objective To understand the carrying rate of Salmonella and analyze of their serotype distribution and drug resistance from egg shell and egg content in commercial eggs. Methods A total of 100 eggs from market and supermarket in Hefei were detected by PCR technology and 14 antibiotics were tested by K-B method based on national standards. Results The detection rate of Salmonella from shell surface was 10% (10/100), and egg contents was 1% (1/100); the 11 isolated strains were identified as S. anatum, and they were all resistance to penicillin, 18.2% of them was resistance to streptomycin, 36.4% of them was medium resistance. Conclusion The carrying rate of Salmonella of egg shell surface was higher than that of egg content from commercial eggs, for antibiotic selection pressure could lead to the difference of the serotype and drug resistance of Salmonella isolates.