气相色谱-质谱法测定醇食品模拟物中 16种多环芳烃

Determination of polycyclic aromatic hydrocarbons in alcohol food simulation by gas chromatography-mass spectrometry

中文关键词:  多环芳烃  气相色谱-质谱法  醇食品模拟物

英文关键词:polycyclic aromatic hydrocarbons  gas chromatography-mass spectrometry  alcohol food simulation

中文摘要:

      目的 建立固相微萃取-气相色谱-质谱联用仪测定醇食品模拟液中16种多环芳烃的分析方法。方法 优化了萃取方式、萃取温度、萃取时间、解吸时间和振荡速率等萃取条件, 样品采用气相色谱-质谱联用仪(gas chromatography-mass spectrometry, GC-MS)总离子流和选择离子模式进行定性定量测定。结果 16种多环芳烃在0.01~10 ?g/L范围内线性良好, R2大于0.99。在0.1、0.5、1.0 μg/L 3个添加水平下, 16种多环芳烃的平均回收率为76.52%?119.8%, 相对标准偏差为0.99%?15.93%。结论 该方法操作简便、灵敏度高、准确度好, 能够满足醇食品模拟物中多环芳烃化合物的检测要求。

英文摘要:

      Objective To develop a method for the determination of polycyclic aromatic hydrocarbons (PAHs) in alcohol food simulation by chromatography-mass spectrometry (GC-MS) using solid phase microextraction (SPME). Methods The experimental conditions of SPME, such as extraction mode, extraction temperature, extraction time, desorption time and agitation speed were optimized. Qualitative and quantitative analysis were carried out by total ion chromatogram and selected ion chromatogram with GC-MS. Results The method showed a good linearity in the range of 0.01~10.00 μg/L for PAHs with R2>0.99. Average recoveries of PAHs were 76.52%~119.8% with relative standard deviation of 0.99%~15.93% at three fortification levels of 0.1, 0.5, and 1.0 μg/L. Conclusion This method is simple, sensitive, and accurate, and it is suitable for the determination of PAHs in 10% alcohol food simulation.