杜仲籽油中不饱和脂肪酸的分离 及其α–亚麻酸含量分析

Separation of unsaturated fatty acid from Eucommia seed oil and the analysis of its α-linolenic acid content

中文关键词:  尿素包合法  不饱和脂肪酸  α–亚麻酸  气相色谱–质谱法

英文关键词:urea addduction fractionation  unsaturated fatty acid  alpha linolenic acid  GC–MS

中文摘要:

      目的 研究获得高含量的不饱和脂肪酸, 尤其是α-亚麻酸的含量。方法 以不饱和脂肪酸碘值为响应值, 采用响应面法对尿素包合法提取杜仲籽油不饱和脂肪酸的工艺进行优化, 并用GC-MS定量分析α-亚麻酸的含量。结果 在脂肪酸、尿素和95%乙醇三者比例为1?3?9.8, 包合温度为?21.8 ℃, 包合时间13 h条件下, 不饱和脂肪酸的碘值达到了289.67 g/100 g, GC-MS定量分析, 其中α-亚麻酸的含量达到85.41%。结论 尿素包合方法可有效地提取不饱和脂肪酸, 并且可以提高α-亚麻酸的含量。

英文摘要:

      Objective To obtain high levels of unsaturated fatty acid, especially of alpha linolenic acid content. Methods Using unsaturated fatty acid iodine value as response value, the response surface methodology was applied to optimize the enrichment of unsaturated fatty acid from Eucommia seed oil by urea adduction fractionation. The content of alpha linolenic acid was analyzed by GC-MS. Results The experimental results showed that the ratio of fatty acid, urea and 95% alcohol was 1?3?9.8, adducting temperature was ?21.8 ℃ and adducting time was 13 h. Under this condition, the unsaturated fatty acid iodine value was 289.67 g/100 g and the content of α-linolenic acid reached 85.41%. Conclusion Extracting unsaturated fatty acid by urea inclusion method is effective, and the content of alpha linolenic acid can be improved.