婴幼儿奶粉中维生素C检测方法的研究

Study on the method to determine vitamin C from infant milk power

中文关键词:  荧光分光光度法  维生素C  婴幼儿奶粉

英文关键词:fluorescence spectrophotometry  vitamin C  infant milk powder

中文摘要:

      目的 应用荧光分光光度计对婴幼儿乳品中维生素C的含量进行测定。方法 以婴幼儿奶粉为例, 依据国家标准GB 5413.18-2010《食品安全国家标准 婴幼儿食品和乳品中维生素C的测定》, 应用日立F-4600型荧光分光光度计测定婴幼儿乳品中维生素C的含量。结果 线性方程为Y=457.79X 28.877, 相关系数为0.9993, 方法检出限为0.1 mg/100 g。在50 mg/100 g、100 mg/100 g 两个加标水平下, 加标回收率为91.8%~101.8%, 相对标准偏差为0.36%~0.85%(n=6)。结论 此方法操作简便, 线性良好是测定食品中维生素C含量的理想方法。

英文摘要:

      Objective To determine vitamin C from infant milk power using Fluorescence Spectrophotometer. Methods Hitachi F-4600 fluorescence spectrophotometer was used to detect vitamin C content in infant milk power according to the national standard “national food safety standards determination of vitamin C in infant foods and dairy” (GB 5413.18-2010). Results The linear equation was Y=457.79X 28.877 with r2=0.9993. The detection limit was 0.1 mg/100 g. The recoveries were 91.8%~101.8% at the spiked level of 50 mg/100 g, and 100 mg/100 g with relative standard deviation of 0.36%~0.85%(n=6). Conclusion The method is sample and effective, and it is a ideal method to determine vitamin C content in foods along with its good linearity.