分光光度法测定芋粉中总还原糖含量

Determination of total reducing saccharide in dry powder of Maranta arundinacea L.by spectrophotomertry

中文关键词:  竹芋  还原糖  分光光度法

英文关键词:Maranta arundinacea L.  reducing saccharide  spectrophotomertry

中文摘要:

      目的 建立竹芋粉中总还原糖含量的 3,5－二硝基水杨酸(DNS)比色测定方法。 方法 以葡萄糖为标 准品, 以 3,5－二硝基水杨酸(DNS)为显色剂, 用分光光度法测定竹芋粉中总还原糖的含量。 结果 在最大吸收 波长为 480 nm, 测得回收率为 98.3%～101.2%, 相对标准偏差为?1.7%, 竹芋粉中总还原糖含量为 77.83%, 竹 芋粉中淀粉含量为 70.05 %。结论 本方法适用于竹芋粉中总还原糖含量的测定。

英文摘要:

      Objective To establish a method for the determination of total content of reducing saccharide in Maranta arundinacea L. Methods Using glucose as standard and DNS as color developing reagent, the content of total reducing saccharide in Maranta arundinacea L. was determined by spectrophotometry. Results The maximum absorption wavelength of the sample was 480 nm. The recoveries were 98.3%～101.2% with the relative standard deviation of 1.7%. The content of total saccharide and starch in Maranta arundinacea L. were 77.83% and 70.05 %, respectively. Conclusion This method is suitable for the detection of total reducing saccharide and starch in Maranta arundinacea L..