木瓜蛋白酶在亲和双水相系统中的分配行为及机制研究进展

Progress on the study of mechanism and partition behavior of papain in affinity aqueous two-phase systems

中文关键词:  木瓜蛋白酶  亲和双水相分配系统  分配行为

英文关键词:papain  affinity aqueous two-phase systems  partition behavior

中文摘要:

      本文查阅相关文献, 对木瓜蛋白酶传统提取方法(超滤法、盐析法、有机溶剂法、亲和层析法)及一些新兴的提取方法如双水相萃取法、亲和双水相法作了简要综述。利用金属螯合亲和双水相分配系统, 能有效获得高纯度木瓜蛋白酶, 但木瓜蛋白酶与亲和成相剂的相互作用及其分配行为与机制尚不清楚。研究木瓜蛋白酶与不同金属离子亲和成相剂之间的相互作用和吸附动力学模型；对酶的亲和结合物进行物理表征和分子模拟。阐明木瓜蛋白酶与不同金属离子亲和成相剂之间的亲和作用原理。将双水相萃取技术和亲和分配技术相融合, 提出基于木瓜蛋白酶的金属螯合亲和双水相分配技术, 该技术有望大力推动木瓜蛋白酶工业化高效制备的发展。

英文摘要:

      The separation methods of papain such as ultrafiltration, salt deposition, solvent extraction, affinity chromatography, aqueous two - phase extraction, affinity aqueous two-phase extraction and so on are evaluated. High purity papain can be expected to obtain by metal chelating affinity aqueous two-phase system (ATPS) technology, but the mechanism and partition behavior of papain in this system are not clear. It is important to study the interactions between papain and different metal ion affinity phase-separating agent, the adsorption kinetics model of papain in ATPS and the physical structure characterization and molecular simulation of the enzyme affinity adsorbent. Metal chelating affinity ATPS technology based on papain separation, will vigorously promote the development of papain industrialization and it has stimulative effect on the development of aqueous two-phase extraction technology.