用湖羊胎替代藏羊胎酶解制备羊胎素工艺研究

Studies on the enzymatic hydrolysis techniques of preparing lamb placenta extract using Hu sheep placenta to replace Tibet sheep placenta

中文关键词:  湖羊胎  藏绵羊胎  酶解；羊胎素  氨基酸组成

英文关键词:Hu sheep placenta (Ovis aries)  Tibet sheep placenta  enzymatic hydrolysis  lamb placenta  amino acid composition

中文摘要:

      目的 对以湖羊胎和传统羊胎素生产原料—藏绵羊胎为原料制备的羊胎素进行比较, 提供湖羊胎替代藏羊胎作为羊胎素生产原料的科学依据。方法 以产于杭嘉湖地区的湖羊胎为原料, 以传统羊胎素生产原料—藏羊胎为对照, 进行制备羊胎素的酶解正交试验。结果 在设定料液比1:10、酶解时间4 h的前提下, 湖羊胎酶解的最佳条件为：温度45 ℃、酶浓度40 U/g、pH值8.0。在此最佳条件下, 湖羊与藏羊胎的水解度分别为35.36%和40.47%, 两种羊胎素冻干粉的产率分别为17.56%、19.44%, 两者的大分子均得到有效降解, 氨基酸组成和含量无明显差异。结论 湖羊胎是一种可以替代藏羊胎的羊胎素生产原料。

英文摘要:

      Objective To compare of the lamb placenta extracts (LPE) prepared by using Hu sheep plecenta and the traditional raw material (Tibet plecenta), So as to provide scientific evidence for using Hu sheep placenta instead of Tibet sheep placenta as the raw material preparing LPE. Methods With the Hu sheep placenta produced in the areas of Hangzhou, Jiaxing and Huzhou as raw material, and the Tibet sheep placenta, the traditional raw material as control, the optimal enzymatic hydrolysis experiments of preparing LPE were conducted. Results The optimal enzymatic parameters were as follows: enzymatic hydrolysis of placenta with 40 U/g tripsin at pH 8.0 and 45 ℃ for 4 h under the designed mixture of one material and ten liquid. Under the optimal condition, the degree of hydrolysis of Hu placenta and Tibet placenta were 17.56% and 19.44%, respectively, the both frozen LPE powder outputs were 35.36% and 40.47%, respectively, and the large molecular proteins of both placentas were degraded into LEP without significant difference in composition of amino acids. Conclusion Hu placenta is one raw material to replace Tibet placenta for preparing LPE.