基于柱切换技术的高压液相色谱法检测奶粉中的维生素D

Detection of vitamin D in milk powder by HPLC based on column-switching system

中文关键词:  阀切换  维生素D  高压液相色谱法

英文关键词:column-switching  vitamin D  high pressure chromatography (HPLC)

中文摘要:

      目的 建立奶粉中维生素D的柱切换式高压液相色谱分析方法。方法 奶粉样品经过皂化处理、石油醚提取、脱水浓缩后, 用柱切换式高压液相色谱仪进行检测。结果 维生素D3在0.2~1.0 ?g/mL范围内线性关系良好, 回归方程Y=1.74181e?006X?0.00662911, 相关系数r=0.9956, 检出限为0.01 ?g/mL, 定量限为0.2 mg/kg, RSD为2.12%, 平均回收率为 96.8%。结论 该方法简单、快速、准确, 为复杂基质食品中低含量物质的检测提供一定参考。

英文摘要:

      Objective To establish a method for the determination of vitamin D in milk powder by high pressure chromatography based on column-switching methods. Methods After saponification treatment, milk powder was extracted with petroleum ether. The extract was dehydrated with anhydrous sodium sulfate, concentrated, and analyzed by HPLC. Results The method showed a good linearity over the range of 0.2~1 μg/mL for vitamin D with r=0.9956. The linear equations was Y =1.74181e-006X-0.00662911 and the average recoveries were 96.8% with the relative standard deviations(RSDs)of 2.12%. The LOD and LOQ were 0.01 μg/mL and 0.2 mg/kg, respectively. Conclusion The method is simply, rapid and accurate, and it provides a reference for the detection of trace substance in complex matrix.