莱克多巴胺ELISA快检技术样品前处理

Study on the sample pretreatment for rapid detection of ractopamine residues in foods by ELISA

中文关键词:  莱克多巴胺  ELISA  样品前处理  基体干扰

英文关键词:ractopamine  ELISA  sample pretreatment  matrix interferences

中文摘要:

      目的 建立适合莱克多巴胺ELISA快速检测技术基体干扰消除的样品前处理方法。方法 样品经乙腈?0.1 mol/L盐酸(84∶16, v/v)处理, 上清液采用正己烷萃取除杂, 水相用NaOH调成碱性后, 用乙酸乙酯萃取, 有机相层吹干后待测。结果 样品采用正己烷萃取1次后, 基体干扰可消除约25%; 萃取3次后, 样品中的基体干扰可被基本消除。结论 该前处理方法能有效消除莱克多巴胺快速检测的基体干扰, 对猪肝、猪肉等肉类食品的适用性良好。

英文摘要:

      Objective To establish an efficient sample pretreatment method for the rapid detection of ractopamine using ELISA technique. Methods The samples were extracted with acetonitrile and 0.1 mol/L HCl (84∶16, v/v), washed with n-hexane and then adjusted to basicity. The extract was further extracted with ethyl acetate and the ethyl acetate extractant was dried for further analysis. The influences of n-hexane, petroleum ether, n-heptane and cyclo-hexane were tested. Results Twenty five percentage of the matrix interferences were eliminated by extracting the sample one time with n-hexane, and the matrix interferences were eliminated after the sample was extracted 3 times. Conclusion This proposed sample pretreatment method is efficient and it has a good potential on the rapid detection of ractopamine in other meat samples.