| Objective Establish an QuEChERS ultra high performance liquid chromatography tandem mass spectrometry method for simultaneous determination of the content of beauvericin (BEA), enniatin A (ENN A), enniatin A1 (ENN A1), enniatin B (ENN B) and enniatin B1 (ENN B1) in chestnuts. Methods Determine the optimal mass spectrometry conditions for 5 target mycotoxins through parameter optimization, select the best liquid chromatographic column and mobile phase through comparative experiments, confirm the optimal extraction reagent and QuEChERS purification column through recovery rate, analyze matrix effect of chestnut on these 5 emerging mycotoxins. Finally, conduct methodological experiments on the established method. Results The method ultimately used acetonitrile- water (80+20) as the extraction reagent for sample pretreatment, selected QuEChERS column (PSA 50mg, MgSO4 150 mg) for cleaning Treatment. The BEH C18 column was used as the chromatographic column and acetonitrile- 2 mmoL/L ammonium acetate was used as the mobile phase. The matrix effect of the method cannot be ignored, the internal standard method should be used for quantitative analysis. The detection limits of BEA、ENN A、ENN A1、ENN B and ENN B1 were 0.03 μg/kg and the quantification limits were 0.1 μg/kg. The recovery rate was between 97.7% and 117.7%, the relative standard deviation was 1.6% to 8.4%, the correlation coefficients were all ≥ 0.9990. Conclusion This method is simple, rapid, with the high accurates and ensitivity, which could be used for simultaneous determination of the content of BEA、ENN A、ENN A1、ENN B and ENN B1 . |
