高效液相色谱法检测不同酶促终止方式茶黄素类物质的浓度

Detection of the concentration of theaflavins with different enzymatic termination methods by high performance liquid chromatography

中文关键词:  茶黄素  HPLC  多酚氧化酶  酶促反应  终止条件

英文关键词:theaflavins  high performance liquid chromatography  polyphenol oxidase  enzymatic reaction  terminal condition

中文摘要:

      目的 分析不同终止方式对茶黄素类物质产量的影响。方法 通过马铃薯多酚氧化酶(polyphenol oxidase, PPO)酶促制备茶黄素, 利用高效液相色谱法(high performance liquid chromatography, HPLC)检测茶黄素类物质, 分析6种不同终止方式对茶黄素类物质产量的影响。结果 经过微波中火、微波高火、100 ℃水浴及3种抑制剂处理后, 分别对茶黄素类物质浓度进行检测, HPLC结果显示100 ℃水浴处理后茶黄素类浓度最高, 其中茶黄素-3,3’-双没食子酸酯(theaflavin-3,3’-gallate, TFDG)的质量浓度为32.47 μg/mL, 茶黄素类物质质量浓度为119.12 μg/mL。结论 经过100 ℃水浴处理后茶黄素类物质的质量浓度最高, 研究结果可对茶黄素的快速检测和工业化生产提供一定的参考价值。

英文摘要:

      Objective To analyze the effects of different termination methods on the yield of theaflavins. Methods Theaflavins were prepared through the enzymatic action of potato polyphenol oxidase (PPO). A high performance liquid chromatography (HPLC) analysis was conducted to detect theaflavin compounds. The effects of 6 kinds of different termination methods on theaflavin yield were then analyzed. Results Following treatment with microwave medium power, microwave high power, a 100 °C water bath and 3 kinds of inhibitors, the concentration of theaflavins was measured. The results of the HPLC analysis indicated that the 100 °C water bath treatment resulted in the highest theaflavin content, with theaflavin-3,3’-gallate (TFDG) reaching 32.47 μg/mL and theaflavin substances at 119.12 μg/mL. Conclusion The concentration of theaflavins is highest after treatment in a 100 °C water bath. The research findings may provide valuable reference for rapid detection and industrial-scale production of theaflavins.