胶体金免疫层析法快速检测草莓中苦参碱残留

Rapid detection of matrine residues in Fragaria ananassa Duch by colloidal gold immunochromatography

中文关键词:  苦参碱  草莓  免疫层析  胶体金

英文关键词:matrine  Fragaria ananassa Duch  immunochromatography  colloidal gold

中文摘要:

      目的 建立胶体金免疫层析法快速检测草莓中苦参碱农药残留分析方法。方法 通过优化金纳米粒子标记参数和试纸条检测线膜浓度, 使胶体金与苦参碱单克隆抗体在最佳条件下结合, 利用抗体和抗原高特异性识别及金标抗体可视化, 从而实现对草莓中苦参碱农药残留的定性检测。结果 确定标记参数为: 1 mL的胶体金溶液加20 μL的0.1 mol/L K2CO3, 抗体8 μg, 选择5%聚乙烯吡咯烷酮(polyvinyl pyrrolidone, PVP)表面活性剂, 抗原划膜质量浓度为0.10 mg/mL; 利用以上条件实现对草莓样本中苦参碱的检出限为10 μg/kg, 并对采集到的126个草莓样本进行快速检测。结论 该方法具有较高灵敏度和特异性, 能够快速准确地检测样本中的苦参碱残留, 可实现现场大量样本的快速筛查。

英文摘要:

      Objective To establish an analytical method for the rapid detection of matrine pesticide residues in Fragaria ananassa Duch by colloidal gold immunochromatography. Methods By optimizing the labeling parameters of gold nanoparticles and the concentration of the test strip detection line, colloidal gold could bind with monoclonal antibodies against matrine under optimal conditions. By utilizing highly specific recognition of antibodies and antigens and visualization of gold labeled antibodies, the localization detection of matrine pesticide residues in Fragaria ananassa Duch could be achieved. Results Determine the labeling parameters as follows: 1 mL of colloidal gold solution with 20 μL of 0.1 mol/L K2CO3, 8 μg of antibody, 5% polyvinyl pyrrolidone (PVP) surfactant, and an antigen transmembrane concentration of 0.10 mg/mL; using the above conditions, the limit of detection of matrine in Fragaria ananassa Duch samples was set to 10 μg/kg, and rapid detection was performed on 126 collected Fragaria ananassa Duch samples. Conclusion This method has high sensitivity and specificity, and can quickly and accurately detect residual matrine in samples, enabling rapid screening of a large number of samples on site.