Fe3O4@SA纳米酶凝胶微球比色法检测植物性食用油过氧化值

Determination of peroxide value in vegetable edible oils by Fe3O4@SA nanozyme gel microspheres colorimetric method

中文关键词:  Fe3O4@SA纳米酶凝胶微球  植物性食用油  比色检测  过氧化值

英文关键词:Fe3O4@SA nanozyme gel microspheres  vegetable edible oil  colorimetric detection  peroxide value

中文摘要:

      目的 制备具有类过氧化物酶活性的Fe3O4@海藻酸钠(sodium alginate, SA)纳米酶凝胶微球, 用于构建检测植物性食用油过氧化值(peroxide value, POV)的比色传感器。方法 通过酶标仪和智能手机采集不同氧化程度油样显色反应后溶液的吸光度值和三原色(red, green, blue, RGB)信息, 建立了不同类型植物性食用油POV的标准曲线, 并评估了检测方法的准确性及实用性。结果 5种类型植物性食用油的POV在0~0.40 g/100 g的范围内呈现良好的线性关系。与国家标准方法的检测结果相比较, 酶标仪比色法和智能手机数字图像比色法的决定系数(determination coefficient, R2)均大于0.9500, 均方根误差(root mean square error, RMSE)均小于0.1。结论 基于Fe3O4@SA纳米酶凝胶微球活性建立的比色检测体系, 具有良好的准确性与实用性, 该研究成果将为食品安全因子的检测提供新思路。

英文摘要:

      Objective To prepare the Fe3O4@SA nanozyme gel microspheres with peroxide-like activity to construct a colorimetric sensor to detect the peroxide value (POV) of vegetable edible oils. Methods The standard curve of POVs of different types of vegetable edible oils was established by collecting the absorbance and the red, green, blue (RGB) information of the solution after color reaction with the microplate reader and smartphone, and the accuracy and practicability of the detection method were evaluated. Results The POVs of 5 kinds of vegetable edible oils showed a good linear relationship in the range of 0–0.40 g/100 g. Compared with the results of the national standard method, the determination coefficient (R2) of the microplate reader colorimetric method and the smartphone digital image colorimetric method were both greater than 0.9500, and the root mean square error (RMSE) was less than 0.1. Conclusion The colorimetric detection system established based on the activity of Fe3O4@SA nanozyme gel microspheres has good accuracy and practicability, and the research results will provide a new idea for the analysis and detection of food safety factors.