变波长高效液相色谱法测定茯苓中17种三萜酸化合物含量

Determination of 17 kinds of triterpenic acid compounds in Poria cocos by variable wavelength high performance liquid chromatography

中文关键词:  茯苓  三萜酸化合物  高效液相色谱法

英文关键词:Poria cocos  triterpenic acid compound  high performance liquid chromatography

中文摘要:

      目的 建立茯苓中17种三萜酸化合物的定量分析方法, 并对不同产地茯苓的三萜酸化合物含量进行分析研究。方法 采用高效液相色谱法, 使用C18色谱柱, 乙腈-0.1%磷酸溶液为流动相梯度洗脱, 流速为1.0 mL/min, 柱温为20 ℃, 进样体积为20 μL, 波长切换的模式测定17种三萜酸化合物含量, 并对测定结果进行分析。结果 17 种三萜酸化合物在各自范围内线性关系均良好(r>0.999), 加标回收率为80.9%~92.5%, 相对标准偏差均小于5.0%, 检出限为0.343~2.839 μg/mL, 定量限为1.143~9.462 μg/mL。结论 建立的高效液相色谱方法样品前处理操作简单, 方法灵敏度高, 稳定性和准确性好, 可用于茯苓中17种三萜酸化合物含量测定。为茯苓的功能性成分开发和利用奠定了研究基础。

英文摘要:

      Objective To establish a quantitative analytical method for 17 kinds of triterpenic acid compounds in Poria cocos and investigate their content in samples from different regions. Methods A high performance liquid chromatography method was developed using an C18 column with gradient elution (acetonitrile-0.1% phosphoric acid solution as the mobile phase). The flow rate was set at 1.0 mL/min, column temperature at 20 ℃, and injection volume at 20 μL. The 17 kinds of triterpenic acid compounds were quantified by wavelength switching mode, and the obtained data were subjected to analysis. Results All 17 kinds of compounds exhibited good linearity (r>0.999) within their respective concentration ranges. The spike recovery rates ranged from 80.9% to 92.5%, with relative standard deviations below 5.0%. The limits of detection and limits of quantitation respectively were 0.343–2.839 μg/mL and 1.143–9.462 μg/mL. Conclusion The established high performance liquid chromatography method features simple sample pretreatment, high sensitivity, excellent stability and accuracy, making it suitable for the determination of 17 kinds of triterpenic acids in Poria cocos. This study provides a foundational basis for the development and utilization of functional components in Poria cocos.