基于粮食检测操作规范性探究影响真菌毒素快速检测法结果准确性的因素

Explore the factors affecting the accuracy of the rapid mycotoxin detection method based on the standardization of grain detection operation

中文关键词:  小麦  玉米  真菌毒素  快速检测  关键操作环节

英文关键词:Triticum aestivum L.  Zea mays L.  mycotoxins  rapid detection  key operational aspects

中文摘要:

      目的 探究影响小麦、玉米真菌毒素快速检测法检测稳定性的过程性因素。方法 以小麦和玉米这两种主流原粮作为研究对象, 系统评价过筛率(99%、97%、95%、93%、91%)、混匀方式(手工、机械单双向)、取样量(300、400、500、600、700 g)及3种市售快速检测仪(赛泰诺、飞测、Charm)对黄曲霉毒素B1、玉米赤霉烯酮和脱氧雪腐镰刀菌烯醇检测结果的影响。结果 原粮中毒素的检测结果与过筛率呈正相关, 最高值为667.61 μg/kg, 但3种毒素变化幅度差异较大, 这可能是由于不同毒素的赋存特性存在差异; 机械双向混匀方式使样品毒素充分混匀, 检测数据误差降低降低至8.1%; 样品质量的增加有助于提高检测结果的稳定性, 但混匀难度增加。此外, 赛泰诺、飞测和Charm 3种快速检测仪在检测毒素时均存在误差, 这可能与操作过程和系统调控有关。结论 优化样品操作步骤, 能够有效提高粮食中真菌毒素快速检测的准确性, 为保障粮食安全和检测效率提供理论依据。

英文摘要:

      Objective To investigate process factors affecting the stability of rapid testing methods for mycotoxins in Triticum aestivum L. and Zea mays L.. Methods To used Triticum aestivum L. and Zea mays L. as primary grain samples, a systematic evaluation was conducted on: Screening rates (99%, 97%, 95%, 93%, 91%) mixing methods (manual, mechanical single/double mixing), sample sizes (300, 400, 500, 600, 700 g), and 3 kinds of commercially available rapid testers (SinoTech, FlyingTest, Charm) on detection results for aflatoxin B1, zearalenone and deoxynivalenol. Results Toxin detection levels in raw grain correlated positively with sieving rate, reached a maximum of 667.61 μg/kg. However, variation among the 3 kinds of toxins differed significantly, likely due to their distinct distribution characteristics. Mechanical bidirectional mixed ensured thorough toxin dispersion, reduced detection error to 8.1%. Increasing sample mass enhanced result stability but complicated mixing procedures. Furthermore, all 3 kinds of rapid testing instruments (SinoTech, FlyingTest and Charm) exhibited measurement errors during toxin detection, potentially attributable to operational procedures and system calibration. Conclusion Optimising sample handling protocols effectively enhances the accuracy of rapid mycotoxin detection in grain, providing a theoretical basis for ensuring food safety and testing efficiency.