微流控技术快速检测食品中病原微生物研究进展

Research progress on rapid detection of pathogenic microorganisms in food by microfluidic technology

中文关键词:  微流控技术  病原微生物  快速检测

英文关键词:microfluidic technology  pathogenic microorganisms  rapid detection

中文摘要:

      食品中的微生物, 尤其是病原微生物, 已对食品安全和人体健康造成了严重影响。近年来, 因病原微生物引起的轻重度传染病事件频频发生, 引起了人们的高度重视。因此, 实现食品中病原微生物的即时分析检测对保障食品安全和人类健康具有重要意义。微流控技术作为一种新兴的检测技术, 凭借微型化、集成化和自动化的优势, 已被广泛应用于食品中病原微生物的快速检测。同时, 其高通量、高灵敏度、高便携性和可重复性检测在实际应用中也表现出巨大潜力。为此, 本文首先总结了不同类型材料(包括无机材料、聚合物材料、纸基材料、复合材料和水凝胶材料)的优势和不足, 以及它们在微流控芯片上的实际应用; 其次, 重点综述了近5年微流控技术在食品中不同病原微生物(包括细菌、真菌、寄生虫和病毒)检测中的应用进展; 最后, 对微流控技术在实际应用检测中存在的不足与挑战做了总结和展望, 旨在为微流控技术在食品安全检测领域的发展提供参考。

英文摘要:

      Microorganisms in food, especially pathogenic microorganisms, have caused serious effects on food safety and human health. In recent years, the occurrence of mild and severe infectious diseases caused by pathogenic microorganisms has attracted people’s attention. Hence, the realization of real-time analysis and detection of pathogenic microorganisms in food is of great significance to ensure food safety and human health. As an emerging detection technology, microfluidic technology has been widely used in the rapid detection of pathogenic microorganisms in food due to its advantages of miniaturization, integration and automation. At the same time, its high throughput, high sensitivity, high portability and repeatability detection also show great potential in practical applications. To this end, this paper summarized the advantages and disadvantages of different types of materials, including inorganic, polymer, paper-based, composite and hydrogel materials, as well as their practical applications on microfluidic chips, secondly reviewed the application progress of microfluidic technology in the detection of different pathogenic microorganisms (including bacteria, fungi, parasites and viruses) in food in the past 5 years. Finally, summarized and prospected the shortcomings and challenges of microfluidic technology in practical application detection, aiming to provide reference for the development of microfluidic technology in the field of food safety testing.